| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170699 |
| Chromosome: | chromosome 10 |
| Location: | 1886145 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTTGCTTGCCGACCAACCCCCACGGCTG |
| Internal bar code: | TCACCACGCCCGAGATGGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.7242 |
| LEAP-Seq n confirming: | 2531 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTCGGGTGAGTAGGTGG |
| Suggested primer 2: | CTATCCTTTGCGCTCTCGAC |