| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170715 |
| Chromosome: | chromosome 15 |
| Location: | 453949 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCCTATCTCAAGGTGGGTGTGCGGGG |
| Internal bar code: | ATACGTTACAGTCTGACGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1181 |
| LEAP-Seq percent confirming: | 98.3594 |
| LEAP-Seq n confirming: | 52639 |
| LEAP-Seq n nonconfirming: | 878 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGCATGATGTGGTTCAG |
| Suggested primer 2: | TCCGCAGAGCAGGACTTTAT |