Insertion junction: LMJ.RY0402.170762_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.170762
Chromosome:chromosome 1
Location:3633605
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g023400 (1 of 1) K14617 - LMBR1 domain-containing protein 1 (LMBRD1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACACACACGCACACCACGCAGGCATAC
Internal bar code:TCCAACCGCATGGGTCCACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:995
LEAP-Seq percent confirming:99.6399
LEAP-Seq n confirming:11346
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTCATGTACCTGATCGGCCT
Suggested primer 2:GAGTCTGTATCCCGCTCTGC