| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170813 |
| Chromosome: | chromosome 17 |
| Location: | 3140902 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721600 | (1 of 1) K12847 - U4/U6.U5 tri-snRNP-associated protein 2 (USP39, SAD1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATGCAGGGCGGTCAGCTGGTCAGGTGTG |
| Internal bar code: | GCTTTATTCTTTGCATTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.1907 |
| LEAP-Seq n confirming: | 3677 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTCAAATGTATGCAGG |
| Suggested primer 2: | AGGGCAAGAAGCAAACTCAA |