Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.170816 |
Chromosome: | chromosome 12 |
Location: | 8608611 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g547200 | PTK6 | Putative tyrosine kinase | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGTAGGCCACGCCTGTTACGCGCGACT |
Internal bar code: | TGACGGTTTCGTTCCCGAAACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 348 |
LEAP-Seq percent confirming: | 88.4894 |
LEAP-Seq n confirming: | 11370 |
LEAP-Seq n nonconfirming: | 1479 |
LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTTCTGGGTTGGTGACAG |
Suggested primer 2: | AGTCCGATTCATCATTTCGC |