| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170836 |
| Chromosome: | chromosome 6 |
| Location: | 2968524 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273200 | (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGCCGTCGTCTAACCCCTCCGCTAGCG |
| Internal bar code: | GGCGGCCAGACCGCGGCCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 96.5051 |
| LEAP-Seq n confirming: | 12564 |
| LEAP-Seq n nonconfirming: | 455 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTGGCAGTCATAGCAC |
| Suggested primer 2: | TAGGTTTTCGCCTGTGCTCT |