| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.170852 |
| Chromosome: | chromosome 8 |
| Location: | 1970138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367800 | NDH2 | Nucleoside diphosphate hydrolase; (1 of 1) 3.6.1.13//3.6.1.22 - ADP-ribose diphosphatase / ADPR-PPase // NAD(+) diphosphatase / NADP pyrophosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGAGGTAAGCGCTGCGAGGCGGCGGG |
| Internal bar code: | CATACTGGATTGTCTTGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 650 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCCCTACCGAGATGGCT |
| Suggested primer 2: | TGCGTCAATCCATTACCAAA |