Insertion junction: LMJ.RY0402.170859_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170859
Chromosome:chromosome 16
Location:1736286
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGTGCCTGACGCAAAGAGGCCAGACCA
Internal bar code:CAGGAACGTAACGGAGTCGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:739
LEAP-Seq percent confirming:96.2581
LEAP-Seq n confirming:1492
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGCAAGGGGCTGTCTTTG
Suggested primer 2:GACTCTGGCTAGCAACGACC