Insertion junction: LMJ.RY0402.170859_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.170859
Chromosome:chromosome 16
Location:1736312
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCCGTGCAGATGTGACCTTGCCTGGTG
Internal bar code:ACGCGCTGCTGGCTGTCGCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:936
LEAP-Seq percent confirming:99.3729
LEAP-Seq n confirming:2377
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCTGGCTAGCAACGACC
Suggested primer 2:CTAGCAAGGGGCTGTCTTTG