Insertion junction: LMJ.RY0402.170859_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170859
Chromosome:chromosome 17
Location:2073342
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g712050 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACTGGCACCTGGCCCTCACTCCTCACTC
Internal bar code:GAAGAGACAGGAGTGTAAAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:511
LEAP-Seq percent confirming:99.8793
LEAP-Seq n confirming:4139
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTCAAGAGCATCACAGCC
Suggested primer 2:ACGGGTACTAGCACCACCAG