Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.170869 |
Chromosome: | chromosome_2 |
Location: | 1399887 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g083354 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGAGTGGTTGTAGGAGGAGGTAGTCCGG |
Internal bar code: | CCTGCCCGCCCGCTTGTTCTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 95 |
LEAP-Seq percent confirming: | 86.4865 |
LEAP-Seq n confirming: | 32 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAGTACTGACTGCGCCGAT |
Suggested primer 2: | TGAGCTCGATCACCTACACG |