| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170941 |
| Chromosome: | chromosome 1 |
| Location: | 4231009 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCATTTGTACTGTCGCCCCCGCGCGTCA |
| Internal bar code: | TGGCTCAAGTACCAGTGGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 99.8387 |
| LEAP-Seq n confirming: | 3713 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTCCTACAGCCAGTCG |
| Suggested primer 2: | CCTGTGCCGTGACAATACAC |