Insertion junction: LMJ.RY0402.170969_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170969
Chromosome:chromosome 9
Location:91001
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTCCACATACACCACCAGGTGCGCGTAC
Internal bar code:TCTCGTCCCCTGATGTCCCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:973
LEAP-Seq percent confirming:97.2245
LEAP-Seq n confirming:1191
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TCGGGAGGGTAGAGTGAGTG
Suggested primer 2:CCTCAACCGTATCTGACCGT