Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.170970 |
Chromosome: | chromosome 3 |
Location: | 2863725 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g162701 | SRH5 | SNF2-related DNA/RNA helicase; (1 of 1) 3.6.4.11 - Nucleoplasmin ATPase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCATACATGCCCCGAATGACCCTTAAA |
Internal bar code: | TCGACACCGCCTGGGCCCAATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 285 |
LEAP-Seq percent confirming: | 98.9305 |
LEAP-Seq n confirming: | 555 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAACCGCACCTCTTATGTT |
Suggested primer 2: | TGGCTACGCCTTGACTTCTT |