| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170976 |
| Chromosome: | chromosome 7 |
| Location: | 3642686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAATCTATCAGGCATTAAAGCTACAACC |
| Internal bar code: | TCACGCCGTAGGGTTTTCTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 99.6623 |
| LEAP-Seq n confirming: | 5312 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGGTGGAGACGTTTGTT |
| Suggested primer 2: | ACCGATAAGCTTAGGGCGAT |