| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170980 |
| Chromosome: | chromosome 7 |
| Location: | 431928 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315250 | CSG5 | Chlamydomonas specific family G protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCCGGTGTCCTCAGCCGTGGTGCGGCA |
| Internal bar code: | GTCTGCGAAGTCTTCATAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 845 |
| LEAP-Seq percent confirming: | 99.7506 |
| LEAP-Seq n confirming: | 1200 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGGTCTTCTCCTCGTG |
| Suggested primer 2: | GAAGGAACGTGCTCTTGGAG |