| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171023 |
| Chromosome: | chromosome 10 |
| Location: | 264148 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419550 | (1 of 1) PTHR28141//PTHR28141:SF1 - FAMILY NOT NAMED // 2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGTGCGCCTGTCGCAGCGGCGTGTGT |
| Internal bar code: | TCAGAGGAATGGCTATGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 96.9455 |
| LEAP-Seq n confirming: | 4126 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCATTAACTTCACGGAC |
| Suggested primer 2: | TACACGGCTGTTGTTTCTGC |