Insertion junction: LMJ.RY0402.171039_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.171039
Chromosome:chromosome 2
Location:3111185
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095058 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCGCGGCCGATGGTTCGCGCTACCAGG
Internal bar code:TCTTCGCATTAGCTGACTCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:704
LEAP-Seq percent confirming:36.658
LEAP-Seq n confirming:1698
LEAP-Seq n nonconfirming:2934
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCGGGCCGTAGGTAGTT
Suggested primer 2:CAGCCTCCCTAGACTGTTGC