| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171043 |
| Chromosome: | chromosome 17 |
| Location: | 7143165 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747647 | (1 of 2) 2.1.1.60 - Calmodulin-lysine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCACAGGTTATCAGCAGTCGATCAGTG |
| Internal bar code: | GCTACATAGTGTCTACGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1152 |
| LEAP-Seq percent confirming: | 99.5556 |
| LEAP-Seq n confirming: | 672 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACCTCTGAACACGCTTT |
| Suggested primer 2: | GTGAATTTCGGTCAGTCGGT |