| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171061 |
| Chromosome: | chromosome 13 |
| Location: | 1227429 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570300 | UBC24 | (1 of 1) K13960 - ubiquitin-conjugating enzyme E2 T (UBE2T, HSPC150); E2 Ubiquitin conjugating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCTGCGTGCAGGCTCTGCGTTGGCTG |
| Internal bar code: | GTACTGGGGGCGGGGTTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCCTGATGGGAGCTTG |
| Suggested primer 2: | CCCCACTTGAGCTTCATCAT |