Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.171074 |
Chromosome: | chromosome_16 |
Location: | 7296890 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g683819 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTAGCAGGGGGAAGGTTAGTTTCGCAATCC |
Internal bar code: | CTTTTACTCCCGTCGCGTGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 401 |
LEAP-Seq percent confirming: | 99.4182 |
LEAP-Seq n confirming: | 4443 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGCATCCTGCTTGTAACTG |
Suggested primer 2: | CTAGCATCTCAGGCTTTGGG |