| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171074 |
| Chromosome: | chromosome 16 |
| Location: | 7296890 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683819 | (1 of 2) PF02900 - Catalytic LigB subunit of aromatic ring-opening dioxygenase (LigB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCAGGGGGAAGGTTAGTTTCGCAATCC |
| Internal bar code: | CTTTTACTCCCGTCGCGTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 99.4182 |
| LEAP-Seq n confirming: | 4443 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCATCCTGCTTGTAACTG |
| Suggested primer 2: | CTAGCATCTCAGGCTTTGGG |