Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.171076 |
Chromosome: | chromosome 17 |
Location: | 2687667 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g717850 | PHC8 | (1 of 1) IPR003882//IPR024616//IPR027417 - Pistil-specific extensin-like protein // Pherophorin // P-loop containing nucleoside triphosphate hydrolase; Pherophorin-chlamydomonas homolog 8 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTCGGCCAGCAGCTCGATGTCCTGGGA |
Internal bar code: | CTCCGAGCAGTCAGGAAGACCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 328 |
LEAP-Seq percent confirming: | 85.5211 |
LEAP-Seq n confirming: | 3922 |
LEAP-Seq n nonconfirming: | 664 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCTCCCCCTCCTCCTTCT |
Suggested primer 2: | GGACTCGAAGTAACGCAAGC |