| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171094 |
| Chromosome: | chromosome 2 |
| Location: | 2483074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092050 | MCP6,MITC6 | (1 of 1) K15121 - solute carrier family 25, member 44 (SLC25A44); Mitochondrial substrate carrier protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGAGGTAAACCTGCAGTGTTGCGTACG |
| Internal bar code: | ATAGCACTTCGGGGTTCACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 89.5804 |
| LEAP-Seq n confirming: | 2519 |
| LEAP-Seq n nonconfirming: | 293 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGAAGGTGGCCACAGAT |
| Suggested primer 2: | TGCATATGTGCAGCTTGTCA |