| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171145 |
| Chromosome: | chromosome 1 |
| Location: | 3159399 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019750 | (1 of 1) PTHR10871:SF4 - DNA TOPOISOMERASE 6 SUBUNIT B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGCGGAAGGGCGAGCTGCTGGGAAGGG |
| Internal bar code: | CGGCCCCGCGAGCATCTCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.7554 |
| LEAP-Seq n confirming: | 2039 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGAAACCAGGGACACG |
| Suggested primer 2: | GTAGCGCAGGATGTAGGAGC |