| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171146 |
| Chromosome: | chromosome 1 |
| Location: | 3383830 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021500 | (1 of 1) K15377 - solute carrier family 44 (choline transporter-like protein), member 2/4/5 (SLC44A2_4_5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTCTTCGCTCTGCGCTGGGTTGATCTC |
| Internal bar code: | GCTGCTGGTGTTCGATAGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 99.5148 |
| LEAP-Seq n confirming: | 2461 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTGTCGTAGGTGCTCTT |
| Suggested primer 2: | ACTTTGCCAACAACTGGGAC |