Insertion junction: LMJ.RY0402.171147_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.171147
Chromosome:chromosome 2
Location:3327716
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095090 (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTATCTGCTTCCGCATTCCATAGCGCCAT
Internal bar code:GACGAGTTAGACGGCCGGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1030
LEAP-Seq percent confirming:98.8814
LEAP-Seq n confirming:2210
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTGCGAGTGAATGAAAC
Suggested primer 2:ACGCTGGCTTGCTTTGTATT