| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171153 |
| Chromosome: | chromosome 7 |
| Location: | 802415 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318250 | HLM12 | (1 of 2) IPR001214//IPR019734 - SET domain // Tetratricopeptide repeat; Histone-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTTCGCAGGCGTCGGCCGCCTCCGAGT |
| Internal bar code: | GGGTGTTGGGGCCGAATTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 99.661 |
| LEAP-Seq n confirming: | 2352 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGATCCTGTGTGGTGATG |
| Suggested primer 2: | TGTCACTACCCAAGGCTTCC |