| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171183 |
| Chromosome: | chromosome 9 |
| Location: | 3295361 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388171 | (1 of 2) PTHR12905//PTHR12905:SF0 - SPERM PROTEIN HOMOLOG // METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGTAATCGGAGGCTTGTTGTCGGCCAT |
| Internal bar code: | GCGAGTGGGACGAAAGGGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 99.2958 |
| LEAP-Seq n confirming: | 846 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTGCGTGAGCTGAATAA |
| Suggested primer 2: | GATGTCAGCCTAGAGACGGC |