| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171252 |
| Chromosome: | chromosome_17 |
| Location: | 3962106 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728700 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTCTTGAAGCTTGGAGGTCAGAAGCTAAA |
| Internal bar code: | AAGCGGATAAGGGGAAGGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 98.7132 |
| LEAP-Seq n confirming: | 537 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGATTATGTTTGGGGG |
| Suggested primer 2: | ATAGGTAGTTGGCAGCCGTG |