| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171254 |
| Chromosome: | chromosome 3 |
| Location: | 5785861 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188750 | HEL21 | (1 of 1) K13177 - ATP-dependent RNA helicase DDX1 [EC:3.6.4.13] (DDX1); DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTATGGCGCAACGGCGCCACGGCAAGTC |
| Internal bar code: | TCGTCGCACGTTTGTTCTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 99.766 |
| LEAP-Seq n confirming: | 1279 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGAATAACCCCTGCACT |
| Suggested primer 2: | ACCCCACATACACGGACAAT |