| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171265 |
| Chromosome: | chromosome 7 |
| Location: | 3912363 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339104 | (1 of 2) 3.6.3.19 - Maltose-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACAATCGCGGGATGCTGTTTTTACCCTC |
| Internal bar code: | CACTCAGAAAAGAAACACGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 790 |
| LEAP-Seq percent confirming: | 98.6925 |
| LEAP-Seq n confirming: | 2189 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTACTCGTATGCGCTGA |
| Suggested primer 2: | AGTAGCGGGGTGTGTGTAGC |