| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171272 |
| Chromosome: | chromosome 3 |
| Location: | 1098558 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148800 | (1 of 1) 2.1.1.4 - Acetylserotonin O-methyltransferase / Hydroxyindole O-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCTGCCACACATTGGACCCGGTCGTC |
| Internal bar code: | AGTACATCTCCATCAAGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 99.4716 |
| LEAP-Seq n confirming: | 1506 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGAGCTTTCCGTGGATG |
| Suggested primer 2: | ATGCAAACGAGCTGTCATTG |