| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171322 |
| Chromosome: | chromosome 17 |
| Location: | 5437847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736900 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAATAAAAGGTGGTTCTACTGGATGCGT |
| Internal bar code: | TGGTCAAATTTCCTCCAACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 99.646 |
| LEAP-Seq n confirming: | 9851 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTTTGGGTCCATGGTGG |
| Suggested primer 2: | TAGCCCTTCCATCCATTCAC |