| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171334 |
| Chromosome: | chromosome 10 |
| Location: | 5984751 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462600 | PUS15 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGCAACATCGGCGATGGCGCTGGAGCCA |
| Internal bar code: | TTCTTTTGGAAGCTGGTGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 99.3978 |
| LEAP-Seq n confirming: | 2806 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGTTGATGTGGTCCAG |
| Suggested primer 2: | GCGACTGCTAGATAGGCACC |