Insertion junction: LMJ.RY0402.171335_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.171335
Chromosome:chromosome 3
Location:2480857
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g159581 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCTCGTGTGACGAACTGGTATGGAATTT
Internal bar code:GGTCCGAGTGGTGCGTTTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:601
LEAP-Seq percent confirming:95.8722
LEAP-Seq n confirming:8733
LEAP-Seq n nonconfirming:376
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCATGACGACTCAGCTTT
Suggested primer 2:CCAGACGCTTTTACAGAGGC