| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171336 |
| Chromosome: | chromosome 8 |
| Location: | 2675775 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373200 | FXL7 | FixL-like PAS domain protein; (1 of 11) PF13426 - PAS domain (PAS_9) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTATGGCGTAGACTGTGGGATTGTGTGGA |
| Internal bar code: | AAGGGTGGCCGTACAGCCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 99.4613 |
| LEAP-Seq n confirming: | 1477 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGTAGGTTGGCAATCAT |
| Suggested primer 2: | ACTTGACGTCCCAACACTCC |