Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.171343 |
Chromosome: | chromosome 7 |
Location: | 5468782 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g350750 | PTO1,PTOX1 | (1 of 2) K17893 - ubiquinol oxidase (AOX1, AOX2); Plastid terminal oxidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGCTTCCTTCCAGGTCTGGCGCTTCG |
Internal bar code: | CCTTCGTTGCCCTATGTGAGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 798 |
LEAP-Seq percent confirming: | 99.5341 |
LEAP-Seq n confirming: | 1709 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTTAACGCTCGCTGTCCTT |
Suggested primer 2: | GTCACGGCTGTATTGGGACT |