| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171362 |
| Chromosome: | chromosome_6 |
| Location: | 1166940 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g257250 | BBS2 | Bardet-Biedl syndrome-2 associated protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGTGTTGAAAGCCGCAGCGCATGGAGGC |
| Internal bar code: | GTCCACGCCATGGGTACTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 99.9266 |
| LEAP-Seq n confirming: | 1362 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGATGTTTGGAAAAGGGG |
| Suggested primer 2: | ACCTACAAACACGAATCCCG |