Insertion junction: LMJ.RY0402.171368_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.171368
Chromosome:chromosome 8
Location:3878383
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g377950 (1 of 3) PF02622 - Uncharacterized ACR, COG1678 (DUF179) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTCGTAATGCTGGGTGCTGGGTGTGCC
Internal bar code:GCTATGTTATGCCAATATGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1255
LEAP-Seq percent confirming:99.4475
LEAP-Seq n confirming:900
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTGCTCAACACACCATC
Suggested primer 2:CATGCAGACGCGGTACTAGA