Insertion junction: LMJ.RY0402.171399_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.171399
Chromosome:chromosome 9
Location:672087
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g403050 LSF2,DSP6 Laforin glucan phosphatase (SEX4)-like 2; (1 of 1) PTHR10159//PTHR10159:SF326 - DUAL SPECIFICITY PROTEIN PHOSPHATASE // LAFORIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGAACATGGGCACAGGTTCGTGGAATG
Internal bar code:CTTGTCTCCAAGATGCGAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:862
LEAP-Seq percent confirming:94.2956
LEAP-Seq n confirming:2992
LEAP-Seq n nonconfirming:181
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACCACCAGCACCAGTA
Suggested primer 2:TGGCATTGAAGCAACAGAAG