| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171430 |
| Chromosome: | chromosome 3 |
| Location: | 4677872 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAATCACCCTCCACAGACGCTGCACCAC |
| Internal bar code: | TCTGAGCTCCGGTGTCGTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 25.2847 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 328 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGACGTTATGCCAGTCCT |
| Suggested primer 2: | TCAGGCACAGACTCATCGTC |