Insertion junction: LMJ.RY0402.171433_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.171433
Chromosome:chromosome_4
Location:2025106
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre04.g218900 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGCTTCGGCGGTGGTGGCGCTGACGGGGG
Internal bar code:CTCGTCTGGGTCAGGGGGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:954
LEAP-Seq percent confirming:62.3235
LEAP-Seq n confirming:971
LEAP-Seq n nonconfirming:587
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GTTATTGCATGACGTGTGCC
Suggested primer 2:CACCAGCAATAACAACACCG