| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171435 |
| Chromosome: | chromosome 2 |
| Location: | 1417838 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083450 | (1 of 1) IPR000104//IPR002938 - Antifreeze protein, type I // FAD-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTAAGACTGAGAGAGGGACGTGCTTAC |
| Internal bar code: | GTCAATGACCGCCGACCATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 75.6839 |
| LEAP-Seq n confirming: | 249 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGTGCGTGCTAACCACT |
| Suggested primer 2: | GTGTGTCTGTGCCATTGACC |