| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171435 |
| Chromosome: | chromosome 2 |
| Location: | 1418031 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083450 | (1 of 1) IPR000104//IPR002938 - Antifreeze protein, type I // FAD-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCCAGCAGGCCGCGTTCGAGCGGCTGT |
| Internal bar code: | CCGCGCAAGGTCCCTCCTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 8.08401 |
| LEAP-Seq n confirming: | 1786 |
| LEAP-Seq n nonconfirming: | 20307 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGTGCGTGCTAACCACT |
| Suggested primer 2: | AGGCCATACATAGCCGTGTC |