| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171436 |
| Chromosome: | chromosome 9 |
| Location: | 2264395 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392652 | (1 of 1) 1.5.1.22 - Strombine dehydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGCTTCATGGCGCTGAGTGCTATCCTA |
| Internal bar code: | CGCGGAGCTCGTTTGTGGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 99.505 |
| LEAP-Seq n confirming: | 11258 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGTCGAAGTGTGCAGG |
| Suggested primer 2: | GACCAGCATGCAGCAGAATA |