Insertion junction: LMJ.RY0402.171450_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.171450
Chromosome:chromosome 2
Location:6359745
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g115400 (1 of 2) IPR002889//IPR008979 - Carbohydrate-binding WSC // Galactose-binding domain-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACTGGTCCATAGGACCCGCGTGCACGGA
Internal bar code:GGACGGTAGCACACCGCTGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:99.8418
LEAP-Seq n confirming:1893
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TCGGACATCAGCAGTAGCAC
Suggested primer 2:CGCCAGAGAACTTTCCGTAG