Insertion junction: LMJ.RY0402.171466_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.171466
Chromosome:chromosome 3
Location:6110517
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g191750 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCATCTAGGCGGGCACAATTTGCGCGAG
Internal bar code:GCTGTACCCTCGTTTCACTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTGCTTCAGCGTCTGTG
Suggested primer 2:GCAAGGGACTCACCATGTTT