Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.171471 |
Chromosome: | chromosome_16 |
Location: | 2994064 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g664500 | BBS3B | Bardet-Biedl syndrome-3 associated protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTACCAGGCCGATGTGGGCCAAAAAGTCTG |
Internal bar code: | GGCCTAATTGCGCGTAAACGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1026 |
LEAP-Seq percent confirming: | 99.7529 |
LEAP-Seq n confirming: | 1211 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGCGTTGTATGGGTCTACT |
Suggested primer 2: | CAAGGTGCCCATCCTGTACT |