| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171471 |
| Chromosome: | chromosome 16 |
| Location: | 2994103 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664500 | ARL6,BBS3B,ARFA1aBBS3B,BBS3 | (1 of 2) K07951 - ADP-ribosylation factor-like protein 6 (ARL6, BBS3); Bardet-Biedl syndrome-3 associated protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCGGCCGCTGGTCGTGTAAGTGTGAA |
| Internal bar code: | TTAATGCTCATTAATTTGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.2337 |
| LEAP-Seq n confirming: | 259 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGTTGTATGGGTCTACT |
| Suggested primer 2: | CAAGGTGCCCATCCTGTACT |