| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171496 |
| Chromosome: | chromosome 1 |
| Location: | 1854122 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010100 | NAT7 | N-acetyltransferase; (1 of 2) 2.3.1.128 - Ribosomal-protein-alanine N-acetyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCTCTAGTAAGGACGTCTTTGTGTCG |
| Internal bar code: | GGTACTCACATATTAGGGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 99.4867 |
| LEAP-Seq n confirming: | 10854 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGTAGGCCACTCTGCAA |
| Suggested primer 2: | TGACTTCTGTGCGGATCAAG |